• Colon Polyp from Min mouse
  • Colon Polyp 2 from Min mouse
  • Aberrant Crypt Foci from Min Mouse
  • Nascent Colon Tumors in AOM/DSS Treated Mice
  • Colon Polyp Cross-Section from AOM/DSS treated mouse
Colon Polyp from Min Mouse
  • Min (Multiple Intestinal Neoplasia) mouse model for intestinal neoplasia - These mice contain a mutation in exon 850 of the APC gene. The mutation causes the formation of many intestinal polyps and less commonly colonic tumors, Making this mouse a useful model for studying intestinal neoplasia.
  • Crypts –The site for stem cells and rapidly proliferating transit amplifying cells
  • Polyp – A tumorous growth projecting from the mucus membrane. It is often found in the gastrointestinal tract.
  • Adenoma Cells – Abnormal, dysplatic cells that may progress to cancer (adenocarcinoma)
  • Dysplastic cells in the colon - Dyplastic cells are immature cells which develop abnormally, resulting in an increased population of abnormal immature cells and a decrease in the population of the mature cells. In the colon, such populations of abnormal cells can form polyps.
  • Muscularis Mucosae – A thin layer of smooth muscle just below the crypts

Colon Polyp 2 from Min Mouse

  • Pathological Cells – Abnormal, diseased cells that may develop into tumors
  • Polyp Stalk – A narrow column of cells that attaches the polyp to the surface of the body tissue

Aberrant Crypt Foci from Min Mouse

  • Aberrant Crypt Foci – Abnormally growing crypts that can be hyperplastic or dysplastic and are believed to be the earliest identifiable stage in polyp formation
  • Hyperplastic vs dysplastic - Hyperplasia refers to the excessive proliferation of cells which can still be regulated by normal control mechanisms. However, dysplasia is the proliferation of abnormal immature cells which can no longer be controlled by normal cellular mechanisms.
  • Goblet Cells – One of three cell types in the colon (the other two are absorptive colonocytes and enteroendocrine cells). Goblet cells secrete mucus to protect and lubricate the epithelial surface

Nascent Colon Tumors in AOM/DSS treated mice

  • AOM (Azoxymethane) - A carcinogen which induces colon cancer in mice
  • DSS (Dextran Sodium Sulfate) - An inflammatory agent which is used to induce in vivo intestinal inflammation in mice.
  • AOM/DSS as a model for colitis associated neoplasia - The combination of AOM and DSS greatly reduces the latency time for the induction of colorectal cancer and induces aberrant crypt foci formation and consequently carcinogenesis. This has become a successful murine model because of its simplicity, affordability, and potency.
  • Inflammatory infiltrate –Dense acute inflammatory infiltrate associated with DSS treatment.
  • Columnar Epithelium – Absorptive and secretory cells that line the surface of the colon
  • Tumor – An abnormal growth of tissue
  • Squamocolumnar Juntion – The junction of rectal stratified squamous epithelium and columnar epithelium
  • Stratified Squamous Epithelium – Flat, layered epithelial cells that line the certain regions of the GI tract that are subject to abrasion
  • Submucosa – A layer of dense connective tissue that joins the smooth muscle (Muscularis Mucosae) to the mucosa
  • Circular Muscle – A layer of muscle that contributes to peristalsis

Colon Polyp Cross-Section in AOM/DSS treated mouse

  • Pathological Cells – Abnormal, diseased cells that may develop into tumors

De Robertis, M., Massi, E., Poeta ML, Carotti S., Morini S., Cecchetelli L., Signori E., & Fazio VM. (2011) The AOM/DSS murine model for the study of colon carcinogenesis: From pathways to diagnosis and therapy studies.

Fawcett, D., & Bloom, W. (1986) A Textbook of Histology.

Amar Patel and John P. Lynch

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